A spectrophotometric method for the determination of reduced glutathione.
نویسندگان
چکیده
In a previous communication (1) it was reported that the products formed by the interaction of fluoropyruvic acid with mercaptans exhibit characteristic ultraviolet absorption spectra. CXor /I-aminothiols with an unsubstituted amino group (cysteine, etc.) gave products of high absorbancy, with a peak in wave length between 295 and 300 rnp (Type II spectrum). Those lacking the free amino group (glutathione, etc.) formed compounds of a much lower absorbancy, with a peak between 265 and 275 rnp (Type I spectrum). This reaction of fluoropyruvic acid was utilized for the quantitative estimation of cysteine and related compounds (2). A similar procedure was not applicable for the direct estimation of glutathione and other thiol compounds possessing Type I spectra because of a relatively low absorption intensity. Further studies revealed, however, that a conversion of the Type I to Type II spectrum can be effected by borate. This effect of borate permitted the development of a spectrophotometric method for the determination of glutathione and related compounds.
منابع مشابه
Determination of mercury (II) by spectrophotometric method based on self assembly of gold nanoparticles
Due to the binding of the –COO- group with the mercury (II), gold nanoparticlesfunctionalized with glutathione can self-assemble to form a supermolecular network in the HAc-NaAc buffer solution. The aggregation of functionalized gold nanoparticles and mercury (II)caused the shift of maximum absorption peak of the UV-Vis spectrum from 520 nm to 609 nm.Based on this principle, a simple spectropho...
متن کاملA simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde
متن کامل
SPECTROPHOTOMETRIC REACTION RATE METHOD FOR THE DETERMINATION OF SLWR BASED ON ITS CATALYTIC EFFEICT ON THE OXIDATION OF GLLOCYANW BY PER0XYDISULPTE
A sensitive and selective procedure was developed for the spectrophotometric determination of silver by its catalytic effect on the oxidation of gallocyanine by peroxydisulphate in acidic media at 540 nm. Silver can be determined in the range of 0.001-0.500 µg/ml with a limit of detection of 0.0005 µg/ml, by a fixed-time method of 4.0 min from initiation of the reaction, The relative standa...
متن کاملKinetic Spectrophotometric Method for Determination of S032- Using Methylen blue
The present work reports a kinetic spectrophotometric method for the determination of traceamounts of sulphite that is based on inhibitory effect of sulphite on the oxidation of Methyleneblue (MB) in acidic media. The reaction is followed spectrophotometrically by monitoring thedecrease in absorbance of MB at 664 nm with a fixed time of 0.5-4.5 minutes. It was found thatsulphite exert a strong ...
متن کاملSensitive and spectrophotometric methods for the determination of Azathioprine in tablets using indigo carmine and methyl orange
A simple, economical, precise and fast visible spectrophotometric method has been developed for the determination of Azathioprine in tablet dosage form. Developed method is based on the formation of extractable colored complex of drug with colouring agent Indigo Carmine and Methyl orange. A wavelength maximum was found to be 683.7 nm and 566 nm. The concentration range of 15-50 μg mL-1 with of ...
متن کاملA Method Based on Ultrasound-assisted Solidification of Floating Drop Microextraction Technique for the Spectrophotometric Determination of Curcumin in Turmeric Powder
A method based on the ultrasound-assisted solidification of floating drop microextraction technique was developed for the spectrophotometric and spectrofluorimetric determination of curcumin in turmeric powder. In this work a small volume of an organic solvent was floated on the surface of an aqueous solution. After sonication the organic solvent is solidified and separated. The effect of extra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of biological chemistry
دوره 233 1 شماره
صفحات -
تاریخ انتشار 1958